An Innovative miR‐Activated Scaffold for the Delivery of a miR‐221 Inhibitor to Enhance Cartilage Defect Repair
نویسندگان
چکیده
The development of treatments to restore damaged cartilage that can provide functional recovery with minimal risk revision surgery remains an unmet clinical need. Gene therapy shows increased promise as innovative solution for enhanced tissue repair. Combination biomaterial approaches led the gene-activated scaffolds capable a sustained delivery therapeutic genes in strictly controlled manner be applied treatment number disorders. Within this study we developed novel microRNA (miR)-activated scaffold mesenchymal stem/stromal cells (MSC) chondrogenesis and repair through inhibitor miR-221 which is known have negative effect chondrogenesis. To fabricate miR-activated scaffolds, composite type II collagen-containing designed specifically were firstly manufactured by lyophilization then functionalised Glycosaminoglycan-binding transduction (GET) system nanoparticles (NPs) encapsulating inhibitor. Subsequently, cultured human-derived MSCs vitro under chondrogenic conditions 28 days. successfully transfected human cargo up silencing using promoted improved more robust cell-mediated response repressed early-stage events related MSC hypertrophy. Taken together, demonstrated capability enhance This thus demonstrates incorporation into might promising approach damage disease. article protected copyright. All rights reserved
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ژورنال
عنوان ژورنال: Advanced therapeutics
سال: 2023
ISSN: ['2366-3987']
DOI: https://doi.org/10.1002/adtp.202200329